Russia has allowed the US and Nato allies to used a country airbase for transits to and from Afghanistan.
Moscow had announced plans to create a Nato transit hub in Ulyanovsk city in March, and the decision was taken on June 25.
A respective decree, signed by Prime Minister Dmitry Medvedev, was published on Friday.
The Nato and Russian officials have sought to allay fears that the hub would turn out to be a full-fledged base.
"We have no intention to establish a base in Russia," the Nato Secretary General Anders Fogh Rasmussen said.
"This is a pragmatic arrangement which allows us to transport non-lethal weapons and troops to benefit our operation in Afghanistan."
Meanwhile, the Russia's foreign ministry said in a statement that: "There never has been and never will be a Nato military base in Ulyanovsk."
The hub would see the "transit of exclusively non-lethal cargoes," he added in the statement.
It comes after Pakistan blocked Nato supplies from crossing its territory after an Nato airstrike that Killed 24 Pakistani soldiers November last year.