Noor Zahi ran the distance in 11.4 seconds and stood in the seventh position in the preliminary heats.
Yousofi ran the distance in 13.29 seconds and stood in the seventh position in the preliminary heats.
Both Afghan athletes will compete in the Olympic Games on Friday afternoon.
Tokyo's 2,848 COVID-19 infections on Tuesday were the Olympic host city's highest since the pandemic began.
The competition will kick off Tuesday at 7:45 am (local Kabul time).
Athletes, the vast majority wearing masks, paraded through an eerily silent National Stadium.
The news is the latest in a series of embarrassments for Tokyo organisers that have sparked outrage at home and abroad…
Five Afghan athletes will represent Afghanistan in four events in the Olympic Games.
Mahdi Yovari, in the shooting category, will also soon arrive in Japan, the National Olympic said.
Organizers confirmed that a visitor from abroad who was working for the Olympics tested positive in a routine check on Friday.
